Composite

Part:BBa_K101007:Design

Designed by: Sarah Hendrickson   Group: iGEM08_Minnesota   (2008-07-28)


TetR and p22 cII Dual Promoter Driving RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the design of this part, the majority of design considerations were dealt with during the construction of the TetR/p22 cII dually repressed promoter (K101002). The promoter design required careful consideration of which operator sites to include, as well as which -35 and -10 sites should be utilized.

A medium strength RBS was chosen to increase the ribosome binding affinity to an appropriate level. Additionally, the decision was made to use only a single, well-characterized terminator, rather than a double terminator.


Source

Constructed from genomic sequences obtained from previously designed BioBrick parts.

References