Regulatory
PN

Part:BBa_K100000:Design

Designed by: Garrett Tobin   Group: iGEM08_PennState   (2008-08-18)

Natural Xylose Regulated Bi-Directional Operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 181
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control.


Source

Taken from E. Coli genome bases 3728830-3729092

References

Song S, Park C (1997). "Organization and regulation of the D-xylose operons in Escherichia coli K-12: XylR acts as a transcriptional activator." J Bacteriol 1997;179(22);7025-32.