Intermediate
RBS-PmeIR

Part:BBa_K093013:Design

Designed by: John Heil, Danielle Nash   Group: iGEM08_Waterloo   (2008-10-29)


PmeI endonuclease with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 670
    Illegal BsaI.rc site found at 363


Design Notes

We wanted to express PmeI. That requires a RBS.


Source

composite part

References