Composite

Part:BBa_K091213:Design

Designed by: Malcolm Campbell   Group: iGEM08_Davidson-Missouri_Western   (2009-02-05)


LacI X86 and pLac promoter upstream of GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2035


Design Notes

We just had to produce the mutant LacI coding sequence but this basic part is already in the registry.


Source

These come from existing parts in the registry. S04174 plus S04165

References