Device

Part:BBa_K091190

Designed by: Xiao Zhu, Alicia Allen, Andrew Gordon   Group: iGEM08_Davidson-Missouri_Western   (2008-08-08)

plux/cI+RBS+lasI+RBS+Mnt+TT+pMnt/lac+RBS+lasI+RBS+cI+TT

This construct uses two hybrid promoters. pLux/cI drives expression of the LasI and Mnt genes. The promoter is designed to respond to activation by LuxR when bound to AI-1 and to repression by lambda cI. pLac/Mnt drives expression of the LasI and cI genes. It is designed for repression by the Lac repressor, which can be derepressed by IPTG, and by the Mnt repressor. LasI is a sythase that produces P-AI-1. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1215
    Illegal XhoI site found at 1177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 306
    Illegal AgeI site found at 1512
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/cellsignalling
//function/regulation/transcriptional
Parameters
None