Device
Part:BBa_K091190
Designed by: Xiao Zhu, Alicia Allen, Andrew Gordon Group: iGEM08_Davidson-Missouri_Western (2008-08-08)
plux/cI+RBS+lasI+RBS+Mnt+TT+pMnt/lac+RBS+lasI+RBS+cI+TT
This construct uses two hybrid promoters. pLux/cI drives expression of the LasI and Mnt genes. The promoter is designed to respond to activation by LuxR when bound to AI-1 and to repression by lambda cI. pLac/Mnt drives expression of the LasI and cI genes. It is designed for repression by the Lac repressor, which can be derepressed by IPTG, and by the Mnt repressor. LasI is a sythase that produces P-AI-1. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1215
Illegal XhoI site found at 1177 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 306
Illegal AgeI site found at 1512 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/cellsignalling
//function/regulation/transcriptional
//function/regulation/transcriptional
None |