Deleted
Sample Discontinued
Experience: Fails
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Composite

Part:BBa_K091189:Design

Designed by: Robert Cool   Group: iGEM08_Davidson-Missouri_Western   (2008-08-08)


pLas/cI+pTet/Mnt+TetR+LasR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 3121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 827
    Illegal BglII site found at 1356
    Illegal BglII site found at 2078
    Illegal BamHI site found at 3061
    Illegal XhoI site found at 1318
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4385
    Illegal AgeI site found at 4582
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no special considerations


Source

Most of the subparts can be found in the registry. The new promoters were built from oligos.

References