Part:BBa_K091150:Design

pLas' Testing Construct C

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 383
  Illegal AgeI site found at 580
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2591
  

Design Notes

pLas' Testing Construct C has the coding region for LasR preceding the region for LuxR, but the order of these regions is reversed in pLas' Testing Construct D.

Source

The sources of the quorum sensing components are the modified lasI regulatory region in P. aeruginosa and the lux components from V. fisheri.