Composite

Part:BBa_K090402

Designed by: Daniel Goodman   Group: iGEM08_Cambridge   (2008-10-29)


B. subtilis Episomal Vector with Constitutive GFP

This episomal vector has within its BioBrick region a constitutively expressed GFP gene. It uses the pUpp strong constitutive promoter and a consensus Bacillus RBS sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5187
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5187
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5193
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5187
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5187
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5187
    Illegal XbaI site found at 5202
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1533
    Illegal BsaI.rc site found at 4261
    Illegal BsaI.rc site found at 6124
    Illegal SapI site found at 3178


[edit]
Categories
//chassis/prokaryote/bsubtilis
//plasmid
Parameters
None