Coding

Part:BBa_K090000:Design

Designed by: Ellis O'Neill   Group: iGEM08_Cambridge   (2008-10-23)

Glutamate gated K+ channel


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 87
    Illegal AgeI site found at 423
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

From peptide sequence of Synechocystis PCC 6803, codon optimised for E.coli, restriction sites removed, and synthesised de novo.


Source

From Genomic sequence

References

Functional characterization of a potassium-selective prokaryotic glutamate receptor: Guo-Qiang Chen, Changhai Cui, Mark L. Mayer and Eric Gouaux [http://www.nature.com/nature/journal/v402/n6763/full/402817a0.html] Mechanisms for ligand binding to GluR0 ion channels: Mark L Mayer, Rich Olson and Eric Gouaux [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WK7-457CY65-9M&_user=1495569&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=1fe249c1818acffada33496175d9c223]