Signalling

Part:BBa_K084012:Experience

Designed by: Yoshimi Iyama, Kohei Kawasaki, Masahiro Tominaga   Group: iGEM08_Chiba   (2008-09-22)

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Applications of BBa_K084012

User Reviews

The iGEM Chiba team conducted tests on this part in the summer of 2008. The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].

One part of the senders cultures were mixed with 1 part of the receiver culture. (Mixed sender culture 500μL with receiver culture 500μL.)

senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by BBa_K084012,BBa_K084008,BBa_K084007 respectively.
senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by BBa_K084012,BBa_K084008,BBa_K084007 respectively.
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iGEMChiba 2008

The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].

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UNIQd2609b1f36a25c87-partinfo-00000001-QINU UNIQd2609b1f36a25c87-partinfo-00000002-QINU