Reporter

Part:BBa_K080010:Design

Designed by: Jennifer Lei   Group: iGEM08_Bay_Area_RSI   (2008-10-29)


puc18-LexAop-eGFP-SV40PA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 407
    Illegal XbaI site found at 401
    Illegal SpeI site found at 3060
    Illegal PstI site found at 3054
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 407
    Illegal SpeI site found at 3060
    Illegal PstI site found at 3054
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 407
    Illegal BglII site found at 3212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 407
    Illegal XbaI site found at 401
    Illegal SpeI site found at 3060
    Illegal PstI site found at 3054
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 407
    Illegal XbaI site found at 401
    Illegal SpeI site found at 3060
    Illegal PstI site found at 3054
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1723
    Illegal SapI site found at 640


Design Notes

puc18 - 193bp-3067bp lexaop - 400bp-194bp eGFP - 3197bp-178bp SV40PA - 3196bp-3066bp


Source

LexA minimal promoter is made synthetically from oligonucleotide synthesis eGFP is pcred from plasmid pWPI SV40PA is from pcDNA3.1 zeo (+)

References