Regulatory

Part:BBa_K080000:Design

Designed by: Jennifer Lei   Group: iGEM08_Bay_Area_RSI   (2008-10-20)

MMP-7 TCFbs minpro - BMP4  Late inducer of Cardiac Muscle Differentiation Cla1(6bp)-Xba1(6bp)-TCFbs minimal Pro (295bp) – pac1 (8bp) – mlu1 (6bp) – kozak (6bp) -BMP4 (1223bp) - Nhe1 (6bp) – sal1 (6bp) = 1562bp

TCFbs minPro was isolated via PCR from a human lung embryonal carcinoma cell line, NT2 genomic DNA (atcc # CRL-1973). 

“Gaire, M., et al. Structure and expression of the human gene for the matrix metalloproteinase matrilysn. (1994) JBC 269:3 2032-2040”

From previous studies, we found a minimal promoter sequence where Beta-catenin/tcf4 could bind and control the expression of MMP7 “Thomas Brabletz, Andreas Jung, Serpil Dag, Falk Hlubek, and Thomas Kirchner. β-Catenin Regulates the Expression of the Matrix Metalloproteinase-7 in Human Colorectal Cancer. 1999. American J. of Path. 155(4):1033-1038”

BMP4 Mus musculus full length cDNA clone from openbiosystems, clone ID# 4219098