Regulatory

Part:BBa_K079018:Design

Designed by: Francesca Ceroni   Group: iGEM08_Bologna   (2008-10-24)

Lac 1 - operator library member


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BBa_K079018 part was designed as the Lac operator 1 sequence flanked by the standard Prefix and Suffix.

Source

Geneart synthesis service.

References

Sadler et al. “A perfectly symmetric lac operator binds the lac repressor very tightly” [Proc. Natl. Acad. Sci. USA Vol. 80, pp. 6785-6789, November 1983]

Lewis et al. "The Lac repressor" [C.R.Biologies 328 (2005)521- 548]

Cox et al. "Programming gene expression with combinatorial promoters" [Molecular Systems Biology 2007]

S Oehler, E R Eismann, H Krämer, and B Müller-Hill "The three operators of the lac operon cooperate in repression."

S Oehler, M Amouyal, P Kolkhof, B von Wilcken-Bergmann, and B Müller-Hill "Quality and position of the three lac operators of E. coli define efficiency of repression."