Regulatory
Lac operat

Part:BBa_K079017:Design

Designed by: Francesca Ceroni   Group: iGEM08_Bologna   (2008-10-24)

Lac symmetric - operator library member


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BBa_K079017 part was designed as the Lac symmetric operator sequence flanked by the standard Prefix and Suffix.


Source

Geneart synthesis service.

References

Sadler et al. “A perfectly symmetric lac operator binds the lac repressor very tightly” [Proc. Natl. Acad. Sci. USA Vol. 80, pp. 6785-6789, November 1983]

Lewis et al. "The Lac repressor" [C.R.Biologies 328 (2005)521- 548]

Cox et al. "Programming gene expression with combinatorial promoters" [Molecular Systems Biology 2007]

S Oehler, E R Eismann, H Krämer, and B Müller-Hill "The three operators of the lac operon cooperate in repression."

S Oehler, M Amouyal, P Kolkhof, B von Wilcken-Bergmann, and B Müller-Hill "Quality and position of the three lac operators of E. coli define efficiency of repression."