Part:BBa_K078008:Design
bphC codes 2,3-Dihydroxybiphenyl-1,2-dioxygenase
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 704
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use this part to construct the degradation pathway of PCBs.
2,3-Dihydroxybiphenyl-1,2-dioxygenase is coded by bphC.This is the second step enzyme of the PCBs deagradation pathway.
This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used. The vector is the standard vector, pSB1AC3, provided by biobrick08. We recommend double digestion of EcoRI+pstI.Digest in NEB Buffer EcoRI + BSA at 37°C for about 3h.
Source
From the PCBs-degrading bacterium Burkholderia xenovorans LB400 chromosome 3.
Type: genomic DNA
References
The biphenyl/polychlorinated biphenyl-degradation locus (bph) of Pseudomonas sp. LB400 encodes four additional metabolic enzymes. Bernd Hofer, Silke Backhaus and Kenneth N. Timmis.Gene, 144 (1994) 9-16