Composite

Part:BBa_K077007:Design

Designed by: Floor Hugenholtz   Group: iGEM08_Groningen   (2008-07-15)


placI and normal RBS with cII behind


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 392


Design Notes

Ligation of BBa_R0010(SpeI and PstI) and BBa_P0453(XbaI and PstI). Plasmid is pSB1A2


Source

BBa_R0010 and BBa_P0453

References