Other

Part:BBa_K076005:Design

Designed by: Sequence from publication (annotated)   Group: iGEM08_Princeton   (2008-10-29)

2A linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is conveniently added by means of specially-designed PCR primers because of its relatively short length (see referenced paper).

Source

This part is described in Osborn et al. "A Picornoviral 2A-Like Sequence-Based Tricistronic Vector Allowing for High-Level Therapeutic Gene Expression Coupled to a Dual-Reporter System." Molecular Therapy, Vol. 12, No. 3, Sept. 2005.

References