Coding

Part:BBa_J92005:Design

Designed by: Timothy Fenton   Group: UC Davis - BIM189C - S10   (2010-05-30)

M. Ruminantium RNA polymerase Subunit A'


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 263
    Illegal NotI site found at 890
    Illegal NotI site found at 2393
    Illegal NotI site found at 2534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 348
    Illegal BglII site found at 807
    Illegal BglII site found at 1095
    Illegal BglII site found at 2616
    Illegal BamHI site found at 1002
    Illegal BamHI site found at 2502
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to make silent mutations at 322, 823, 2137 to get rid of PstI site.

Source

This comes from a genomic sequence

References