Coding

Part:BBa_J78000:Design

Designed by: Nathan Dunn   Group: Neilan lab   (2009-08-25)


Microcystin Synthetase mcyC CATTe (Leucine, Macrocyclisation)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3618
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3618
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3618
    Illegal BglII site found at 895
    Illegal BamHI site found at 1626
    Illegal XhoI site found at 3689
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3618
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3618
    Illegal AgeI site found at 2376
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contains EcoRI site, suffix assembly only


Source

Microcystis aerugionsa PCC 7806

References