Plasmid_Backbone

Part:BBa_J72209:Design

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)


kanamycin + ampicillin resistant p15A vector for 2ab assembly


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3329
    Illegal PstI site found at 2156
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3329
    Illegal NheI site found at 1015
    Illegal PstI site found at 2156
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a suffix.
    Illegal BamHI site found at 1
    Illegal XhoI site found at 2196
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3329
    Illegal PstI site found at 2156
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3329
    Illegal PstI site found at 2156
    Illegal AgeI site found at 605
    Illegal AgeI site found at 929
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1297


Design Notes

n/a


Source

n/a

References