Coding

Part:BBa_J72040:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)


{Bmll6} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Polymerase Chain Assembly

Gene Synthesis of Bmll16 {rbs_at_10000.Med21DC1-132>}

Pool mll16 through mll33, assemble by PCA
PCR mll16/mll33 on PCA reaction        (457, EcoRI/BamHI
Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll16   {rbs_at_10000.Med21DC1-132>}
----
mll16     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
CCATAGAATTCATGAGATCTGAATACCCTACTAAGGACATTC
mll17     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
GTCAGACGGTCGGTCATTTTCTGGCGAATGTCCTTAGTAGGGTAT
mll18     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
AAAATGACCGACCGTCTGACCCAACTCCAAATTTGCCTGGACCAG
mll19     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
GGGTAGCGCAGAACTGTTCGGTCATCTGGTCCAGGCAAATTTGGA
mll20     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
CGAACAGTTCTGCGCTACCCTGAACTACATCGACAAAAACCACGG
mll21     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
TTCGTTAACGGTCAGACGTTCGAAACCGTGGTTTTTGTCGATGTA
mll22     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
AACGTCTGACCGTTAACGAACCGCAGATGTCTGACAAACACGCTA
mll23     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
GAAAACTCTTCCGGCGGAACAACGGTAGCGTGTTTGTCAGACATC
mll24     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
GTTCCGCCGGAAGAGTTTTCTAACACTATTGACGAACTCTCCACC
mll25     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
TCTGACGGGTTTTCAGGATGATGTCGGTGGAGAGTTCGTCAATAG
mll26     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
CATCCTGAAAACCCGTCAGATCAACAAACTGATCGACTCTCTGCC
mll27     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
TTCCTCTGCGGAAACGTCAACGCCTGGCAGAGAGTCGATCAGTTT
mll28     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
TTGACGTTTCCGCAGAGGAACAACTCCGTAAAATCGACATGCTCC
mll29     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
TCTTCAACTTCAACGAGCTTTTTTTGGAGCATGTCGATTTTACGG
mll30     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
AAGCTCGTTGAAGTTGAAGACGAAAAAATCGAAGCTATCAAAAAA
mll31     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
CAACGTGACGCAGCAGTTTTTCTTTTTTTTTGATAGCTTCGATTT
mll32     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
AAAACTGCTGCGTCACGTTGACTCTCTGATCGAAGACTTCGTTGA
mll33     PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
CGTTAGGATCCACCGTCAACGAAGTCTTCGATCAG

References