Coding
Part:BBa_J72039:Design
Designed by: Jennifer Brophy Group: Anderson Lab (2010-07-15)
{Bmll3} in BBb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
Polymerase Chain Assembly
Gene Synthesis of Bmll3 {rbs_at_10000.MED7DNDC102-205>}
Pool mll4 through mll15, assemble by PCA PCR mll4/mll15 on PCA reaction (384, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll3 {rbs_at_10000.MED7DNDC102-205>} ---- mll4 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) CCATAGAATTCATGAGATCTCAGCTAAAATCAACGTATTAAG mll5 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) TTTCGGTAGATTTTTTCATCGCTATGGAGTGCCTTAATACGTTGATTTTAGC mll6 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) GATGAAAAAATCTACCGAAAACGAATCTACCAACTACCAGTACAAAATCCAG mll7 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) AGCAGCAGAGATTTCAGCAGTTTACGCAGTTCCTGGATTTTGTACTGGTAGT mll8 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) CTGCTGAAATCTCTGCTGCTGAACTACCTGGAACTGATCGGTGTTCTGTCTA mll9 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) TTCAACTTTACGTTCGTACATGTCCGGGTTGATAGACAGAACACCGATCAGT mll10 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) TGTACGAACGTAAAGTTGAAAACATCCGTACCATCCTGGTTAACATCCACCA mll11 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) GAGACTGGTGCGGACGGTATTCGTTCAGCAGGTGGTGGATGTTAACCAGGAT mll12 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) ATACCGTCCGCACCAGTCTCGTGAATCTCTGATCATGCTGCTGGAAGAACAG mll13 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) ATTTCACGGATTTCACCACGTTTGTATTCCAGCTGTTCTTCCAGCAGCATGA mll14 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) CGTGGTGAAATCCGTGAAATCGAACAGGTTTGCAAACAGGTTCACGACAAAC mll15 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) CGTTAGGATCCTTAAGAGGTCAGTTTGTCGTGAACCTGTTTG