Coding

Part:BBa_J72039:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)


{Bmll3} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Polymerase Chain Assembly

Gene Synthesis of Bmll3 {rbs_at_10000.MED7DNDC102-205>}

Pool mll4 through mll15, assemble by PCA
PCR mll4/mll15 on PCA reaction        (384, EcoRI/BamHI
Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll3   {rbs_at_10000.MED7DNDC102-205>}
----
mll4     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
CCATAGAATTCATGAGATCTCAGCTAAAATCAACGTATTAAG
mll5     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
TTTCGGTAGATTTTTTCATCGCTATGGAGTGCCTTAATACGTTGATTTTAGC
mll6     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
GATGAAAAAATCTACCGAAAACGAATCTACCAACTACCAGTACAAAATCCAG
mll7     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
AGCAGCAGAGATTTCAGCAGTTTACGCAGTTCCTGGATTTTGTACTGGTAGT
mll8     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
CTGCTGAAATCTCTGCTGCTGAACTACCTGGAACTGATCGGTGTTCTGTCTA
mll9     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
TTCAACTTTACGTTCGTACATGTCCGGGTTGATAGACAGAACACCGATCAGT
mll10     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
TGTACGAACGTAAAGTTGAAAACATCCGTACCATCCTGGTTAACATCCACCA
mll11     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
GAGACTGGTGCGGACGGTATTCGTTCAGCAGGTGGTGGATGTTAACCAGGAT
mll12     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
ATACCGTCCGCACCAGTCTCGTGAATCTCTGATCATGCTGCTGGAAGAACAG
mll13     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
ATTTCACGGATTTCACCACGTTTGTATTCCAGCTGTTCTTCCAGCAGCATGA
mll14     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
CGTGGTGAAATCCGTGAAATCGAACAGGTTTGCAAACAGGTTCACGACAAAC
mll15     PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3)
CGTTAGGATCCTTAAGAGGTCAGTTTGTCGTGAACCTGTTTG

References