Coding

Part:BBa_J72033:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)


{Bmll70} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll70 {<Strep!}

Wobble mll70/mll71                (61, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll70         {<Strep!}
----
mll70    Forward wobble for {<Strep!} (Bmll70)
CCATAGAATTCATGAGATCTacctggagccacccgcagttcgaa
mll71    Reverse wobble for {<Strep!} (Bmll70)
CGTTAGGATCCttatttttcgaactgcgggtgg


References