Part:BBa_J72019:Design

{Bmll54} in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll54 {rbs_at_10000.FLAG>}

Wobble mll54/mll55                (97, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll54         {rbs_at_10000.FLAG>}
----
mll54    Forward wobble for {rbs_at_10000.FLAG>} (Bmll54)
CCATAGAATTCATGAGATCTGGGACAATCCCGGTCTTTAAGAACAGGAATTACATGTCTGAC
mll55    Reverse wobble for {rbs_at_10000.FLAG>} (Bmll54)
CGTTAGGATCCCTTGTCGTCGTCATCCTTGTAATCGTCAGACATGTAATTCCTG