Coding

Part:BBa_J72017:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)

{Bmll38} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll38 {rbs_at_10000.AP>}

Wobble mll38/mll39                (108, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll38         {rbs_at_10000.AP>}
----
mll38    Forward wobble for {rbs_at_10000.AP>} (Bmll38)
CCATAGAATTCATGAGATCTCACAAACTGATACAAACGGCGGGGAAATCATGGGCCTGAACGATATTTTTG
mll39    Reverse wobble for {rbs_at_10000.AP>} (Bmll38)
CGTTAGGATCCTTCATGCCATTCAATTTTCTGCGCTTCAAAAATATCGTTCAGGCCCATG


References