Coding

Part:BBa_J72010:Design

Designed by: John Anderson   Group: Anderson Lab   (2008-07-16)


{lacZ>} lacZ basic part with no stop in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR ca1133F/ca1134R on MG1655 genomic DNA  (3065, BglII/XhoI)
Sub into pBca9145-Bca1089                  (BglII/XhoI)
Product is pBca9145-Bca1133
----
ca1133F	LacZ eXtreme with start no stop
ctctgAGATCTATGACCATGATTACGGATTC
ca1134R	Removing EcoRI in lacZ
CTGGTAATGGTAGCGACCGGCGCTCAGCTGaAATTCCGCCGATAC

References