Coding
Part:BBa_J72010:Design
Designed by: John Anderson Group: Anderson Lab (2008-07-16)
{lacZ>} lacZ basic part with no stop in BBb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
PCR ca1133F/ca1134R on MG1655 genomic DNA (3065, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI) Product is pBca9145-Bca1133 ---- ca1133F LacZ eXtreme with start no stop ctctgAGATCTATGACCATGATTACGGATTC ca1134R Removing EcoRI in lacZ CTGGTAATGGTAGCGACCGGCGCTCAGCTGaAATTCCGCCGATAC