Composite

Part:BBa_J72002:Design

Designed by: John Anderson   Group: Anderson Lab   (2008-07-16)

{CmR} Chloramphenicol resistance gene in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR ca1121R/ca1172R on pBca1121  (920bp, BglII/XhoI)
Sub into pBca9145-Bca1144#5      (BglII/XhoI, 2063+910, L)
Product is pBca9145-Bca1172
----
ca1121R	EcoRI/BglII cmR cassette
gcaatAGATCTttgatcgggcacgtaagagg
ca1172R	Biobrickin CmR extreme basic part
gcaaaCTCGAGttaGGATCCgaggcttggattctcacc

References