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Part:BBa_J70635:Design

Designed by: Tom Knight   Group: Knight Lab   (2010-11-01)


Downstream genomic DNA fragment of Mesoplasma florum for recombination experiment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no biobrick restriction enzyme sitss, primer binding


Source

PCR from the M. florum genome. Primers:

  • ttccgaattcgcggccgcttctagag gagattattatggcaaaag,Mfl2I-dn-F
  • ttccactagtagtaatatgagtaaattcac,Mfl2I-dn-R


References