Part:BBa_J70634:Design

Upstream genomic DNA fragment of Mesoplasma florum for recombination experiment

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Avoidance of biobrick restriction sites, possible primer binding sites.

Source

PCR from the M. florum genome. Primers:

• ttccgaattcgcggccgcttctagag tccaaatagtgtggatac,Mfl2I-up-F
• ttccactagtaaagacatctatgtttttttac,Mfl2I-up-R