Tag

Part:BBa_J70624:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-08-08)

RFC12 FLAG Tag Head Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Oligos used: 

AATTC GCGGCCGC T ACTAGT ATGAAAGACTATAAGGATGACGATGACAAA GCTAGC A GCGGCCG CTGCA, FLAG-F

GCGGCCGCTGCTAGCTTTGTCATCGTCATCCTTATAGTCTTTCATACTAGTAGCGGCCGCG, FLAG-R


Source

Synthesized

References

<biblio>

  1. Kimple pmid=18429272
  2. Terpe pmid=12536251

</biblio>