Tag

Part:BBa_J70620

Designed by: Joseph Lynch   Group: Knight Lab   (2010-08-05)

RFC12 Maltose Binding Protein Internal Domain [malE]

This Maltose-binding protein is designed for either N-terminal or C-terminal fusions. MBP fused proteins can be purified in one step affinity chromatography using a cross-linked amylose matrix and then eluted with 10 mM maltose. Note that the amylose matrix can only be regenerated, in most cases, up to five times. The large size of this tag can also affect the fused protein.

MBP fusions often have higher levels of expression and solubility that the native untagged protein, with typical yields of up to 40 mg/liter culture.

Usage and Biology

Common tag information:

  • Matrix: Cross-linked Amylose
  • Yield/Purity: High yield, >70% purity
  • # Steps: One step
  • Fusion Location: Internal
  • Affects Function?: Possibly

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 55


[edit]
Categories
//proteindomain/affinity
//proteindomain/internal
Parameters
None