Coding

Part:BBa_J70602:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-06-28)

VioD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 928
    Illegal SapI.rc site found at 1003


Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatgaagattctggtcattggtgctgg l:54 P(g/b):g Ho(max#):-109.4 Het(max#):-115.82 Tm:69.6 GC(%):50

Reverse primer: gtttcttcctgcagcggccgctactagtattattaagcgctgcaaagcataacgcag l:57 P(g/b):b Ho(max#):-115.82 Het(max#):-115.82 Tm:69.8 GC(%):49.1

Source

designed

References