Generator

Part:BBa_J70597:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-06-27)

J70577 cut with BsaXI and with 6his inserts (J70564-fi) added


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 907
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 836


Design Notes

Look at part J70575


Source

Created by cutting part J70577 with BsaXI and adding glycine-serine-6 his inserts (legacy from prior design)

forward (J70562-fi): aaGGATCCCACCACCACCACCACCACTAATAAtactagtagcggc

reverse (J70562-ri): gctactagtaTTATTAGTGGTGGTGGTGGTGGTGGGATCCtttgt

References