RBS

Part:BBa_J70591:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-06-18)

RFC12 Elowitz RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with synthetic oligos: 

5' AATTC GCGGCGC T ACTAGT AAAGAGGAGAAA GCTAGC A GCGGCCG CTGCA 3', RBS-F

5' GCGGCCGCTGCTAGC TTTCTCCTCTTT ACTAGTAGCGCCGCG 3', RBS-R

Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

Source

Part BBa_B0034

References