Part:BBa_J70583:Design

AcKRS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 397
  Illegal BglII site found at 758
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 93
  Illegal BsaI.rc site found at 131
  

Design Notes

none

Source

for A Convenient Method for Genetic Incorporation of Multiple Noncanonical Amino Acids into One Protein in Escherichia Coli Ying Huang, William K. Russell, Wei Wan, Pei-Jing Pai, David H. Russell, and Wenshe Liu*