Temporary

Part:BBa_J70556:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-02-17)

BsaXI test circuit: J70555.J70554 or J70554.J70550.J70554


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1494
    Illegal AgeI site found at 1606
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1974


Design Notes

Assembly


Source

Assembly

oligonucleotide part to insert after cutting with BsaXI

forward fusion (J70556-f): aaggatctggatctggatctaAGA

reverse fusion (J70556-r): atctaggtctaggtctaggaaCAA

forward (deadfusion J70556d-f): AAtaataaTACTAatgAAGA

reverse (deadfusion J70556d-r): TCATTAGTATTATTATTGTT

References