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Part:BBa_J70509:Design

Designed by: Tom Knight   Group: Knight Lab   (2009-10-03)


Mesoplasma florum origin of replication


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None. Modified base in the 5' end of the binding region of the F3 primer eliminates an EcoRI site.



Source

PCR from genomic DNA

References