Temporary

Part:BBa_J70498:Design

Designed by: Julie Norville   Group: Knight Lab   (2009-09-07)


Red {-1,4;0,5} PsrI part (for non protein/AG parts)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 795
    Illegal AgeI site found at 907
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AG on left side; similar to J70394

pcr:

Forward primer: gccgcttctagag gatacat gaac atg caatacgcaaaccgcctctcc

reverse primer: gtttcttcctgcagcggccgctactagtagactctagtagagagcgttcaccgac



Source

designed

References