Generator
Part:BBa_J70304
Designed by: Tom Knight Group: Knight Lab (2009-05-06)
constitutive cat cassette for Mesoplasma florum
This part is a version of J70026, the cat cassette from pC194. The original cassette is inducible, with a 5' UTR containing a hairpin structure which sequesters the Shine-Dalgarno sequence. In this mutation, we eliminate the hairpin structure from the 5' UTR to attempt a constitutive construct.
This modified cassette is functional at 15 ug/ml chloramphenicol in Top10 E. coli cells. Slow growth and small colonies are evident on normal 35 ug/ml chloramphenicol plates.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/prokaryote/mflorum
None |