Part:BBa_J70030:Design

A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 43
  

Design Notes

Changed MabI format to be compatible with BBa_J70012

Can be used to removed protein tails for protein fusions, see BBF RFC15

TACTAGAG and TACAGAT represent sequences for BioBrick scars that are created when

two BioBrick parts are fused together.

^s represent restriction enzyme cut sites.

General Function

XXXXX XXXXX^ XXX  TACTAGAG  BioScaffold Part BBa_J70030 TACTAGAT XXXXX^XXXXX
XXXXX^XXXXX  XXX  ATGATCAC                              ATGATCTA^XXXXX XXXXX

Function when the left part is a protein part.

XXXXX XXXXX^ TAA  TACTAGAG  BioScaffold Part BBa_J70030 TACTAGAT XXXXX^XXXXX
XXXXX^XXXXX  ATT  ATGATCAC                              ATGATCTA^XXXXX XXXXX

What happens after cutting with MabI or PacI and purifying with either gel purification

or a Qiagen PCR cleanup spin column.

General Case

XXXXX XXXXX^                                                    ^XXXXX
XXXXX^                                                    ^XXXXX XXXXX

Case when left part is a protein part

XXXXX XXXXX^                                                    ^XXXXX
XXXXX^                                                    ^XXXXX XXXXX

Source

this part is designed