Part:BBa_J70026:Design
cat cassette (chloramphenicol resistance)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mutation in sequence at base 99, ignored. GATC site eliminated at the end by truncation.
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGaatagttacccttattatcaag,J70026-F
GTTTCTTCCTGCAGCGGCCGCTACTAGTAtctggagctgtaatataaaaacc,J70026-R
Source
Genbank V01277, by way of pKV104 from Duncan Krause. Bases 978-2006; missing T in the Krause sequence at base 99 of this sequence.
References
Hahn TW, Mothershed EA, Waldo RH 3rd, Krause DC. Construction and analysis of a modified Tn4001 conferring chloramphenicol resistance in Mycoplasma pneumoniae. Plasmid. 1999 Mar;41(2):120-4. PMID: 10087215
Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J Bacteriol. 1982 May;150(2):815-25. PMID: 6950931