Coding
Part:BBa_J70024:Design
Designed by: Tom Knight Group: Knight Lab (2008-10-20)
TetM gene, recoded compromise E. coli & Mesoplasma florum
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Elimination of restriction sites, CGG codons, UGA codons, rare codons in both species.
GTTTCTTCGAATTCGCGGCCGCTtctagatgaaaatcataaatatcgg,J00024-F
GTTTCTTCCTGCAGCGGCCGCTactagtattattatgtgattttgttg,J00024-R
Source
Synthesized from the Ureaplasma urealyticum tetM gene sequence, with recoding. Genbank P09757.
References
Sanchez-Pescador,R., Brown,J.T., Roberts,M. and Urdea,M.S. The nucleotide sequence of the tetracycline resistance determinant tetM from Ureaplasma urealyticum Nucleic Acids Res. 16 (3), 1216-1217 (1988) PMID: 3344217