Plasmid
GENEART pG

Part:BBa_J70022

Designed by: Reshma Shetty   Group: Knight Lab   (2008-09-25)

GENEART pGA4 plasmid

The sequence of the plasmid that contains BBa_I712016.

Cloning plasmid used by Geneart for iGEM parts. This plasmid does not have a standard BioBrick cloning site.

Geneart pGA4.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 36
    Illegal EcoRI site found at 2958
    Illegal XbaI site found at 2973
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal PstI site found at 42
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 36
    Illegal EcoRI site found at 2958
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal PstI site found at 42
    Illegal NotI site found at 8
    Illegal NotI site found at 72
    Illegal NotI site found at 2964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 36
    Illegal EcoRI site found at 2958
    Illegal BamHI site found at 54
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2958
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 36
    Illegal PstI site found at 42
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2958
    Illegal EcoRI site found at 36
    Illegal XbaI site found at 2973
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal PstI site found at 42
    Illegal NgoMIV site found at 2624
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 365


[edit]
Categories
//plasmidbackbone/synthesis
Parameters
None