Coding

Part:BBa_J64978:Design

Designed by: Kristopher Kuchenbecker   Group: Voigt Lab   (2007-03-25)


NRII2302


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 934
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to remove the effect of cellular nitrogen levels on the glnG transcription enhancer circuit, this mutant glnL can be incorporated to ensure activation of glnG effector molecules.


Source

E. coli K12

References