Part:BBa_J64804

The promoter region (inclusive of regulator binding sites) of the B. subtilis RocDEF operon

This is the reported promoter region from the Bacillus subtilis RocDEF operon as taken from the DBTBS website (http://dbtbs.hgc.jp/). Base 1 to base 21 contains the binding site for the binding factor, RocR. The sequence, TATGCAAAAGAATTTTGCACT, reportedly contains the consensus sequence for binding of this factor. Base 42 to base 62 contains another binding site for the RocR binding factor, with ATATCAGAATGTTTTTGCACC as the binding consensus for this sequence. Bases 113-135 outline the binding site of the binding factor AhrC (CTTGCATTTATATAAAGGGAAAG). Bases 96 to 135 outline the promoter region for this operon (CTTGATTTGGCACAGAACTTGCATTTATATAAAGGGAAAG).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 91
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 91
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 91
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 91
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 91
  
• 1000
  COMPATIBLE WITH RFC[1000]