Regulatory

Part:BBa_J64800:Design

Designed by: laura lavery   Group: Voigt Lab   (2007-03-24)


RHLR/RHLI Inducible & LasR/LasI repressible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a 17bp spacer between the -35 and -10 regions and the binding site for LasR/LasI is 20bp long. I was able to fit the whole binding site in the spacer region because the last three base pairs of the -35 region and the first three base pairs of the LasR/LasI binding site are the same.

Source

References