Part:BBa_J63008

SV40 nuclear localization sequence from SV40; yeast codon optimized

yeast codon-optimized nuclear localization sequence from simian virus 40

IISER_BHOPAL 2021

Usage and Biology

The nuclear import of plasmid DNA mediated by NLS from SV40 T antigen is a two-step process. The first step involves targeting of import substance to the cytoplasmic side of the nuclear membrane. The process is energy independent and can occur at 0°C. The second step is translocation which is energy and temperature-dependent. It has been found that chelating cytosolic Ca2+ with BAPTA inhibit the DNA-NLS import, and DNA-NLS import is competitively inhibited by BSA-NLS conjugates, reinforcing the specificity of NLS in directing the nuclear import of DNA-NLS complexes[1].

References

1.Collas, P., & Aleström, P. (1996). Nuclear localization signal of SV40 T antigen directs import of plasmid DNA into sea urchin male pronuclei in vitro. Molecular Reproduction and Development, 45(4), 431–438.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]