Plasmid

Part:BBa_J61149

Designed by: John Anderson   Group: Arkin Lab   (2007-09-04)

pBca1101 RIPS reporter plasmid in BBb

pBca1101 is a pUC/Amp plasmid in BBb format. It deviates from pBca9145 by inserting an RFP cassette between the BamHI and XhoI sites. When a RIPS-generating part is placed between the BglII and BamHI sites, it acts as an RFP reporter.

An annotated version of the sequence is here. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 2905
    Illegal BamHI site found at 1
    Illegal XhoI site found at 851
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
    Illegal AgeI site found at 561
    Illegal AgeI site found at 673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1944


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