Intermediate

Part:BBa_J61029:Design

Designed by: John Anderson   Group: Arkin Lab   (2006-09-21)


[Tnp1-PN][oriTR]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1220
    Illegal PstI site found at 885
    Illegal NotI site found at 798
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1720
    Illegal XhoI site found at 690
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 885
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 1858
    Illegal NgoMIV site found at 1868
    Illegal AgeI site found at 1208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1352
    Illegal BsaI.rc site found at 1173
    Illegal SapI site found at 309
    Illegal SapI site found at 1760
    Illegal SapI.rc site found at 621


Design Notes

J61023 J01003


Source

standard biobrick assembly

References