Reporter

Part:BBa_J54103:Design

Designed by: Yusaku Nakashima   Group: iGEM06_Tokyo_Alliance   (2006-10-17)


Promoterless-GFP(A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 27
    Illegal BamHI site found at 15
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 714


Design Notes

We use this as negative control. This prasmid is the basic part to insert one or several protein_binding_sites.

Source

iGEM part BBa_I7100. We deleted the promoter and inserted newly designed prefix and suffix each of which has 2 restriction-enzyme-sites(prefix: SalI and BamHI,surfix: BglII and MulI) But we did not insert new promoter.

References